TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate
##plugins.themes.academic_pro.article.sidebar##
Published
Feb 27, 2024
Download
Statistic
Downloads
Download data is not yet available.
##plugins.themes.academic_pro.article.main##
Abstract
Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule
##plugins.themes.academic_pro.article.details##
How to Cite
Sibuea, C. V., Sitanggang, E. J., Simaremare, A. P., Silaen, R. T., Kuara , G., Samosir, S. C., Ginting, K. M. M., Yana, H. Y., Pratama, G., Mutiara, M. and Angeline, W. K. (2024) “TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate”, Science Midwifery, 11(6), pp. 939-944. doi: 10.35335/midwifery.v11i6.1427.
References
Altaie, A., Owston, H., & Jones, E. (2016). Use of platelet lysate for bone regeneration-are we ready for clinical translation? World Journal of Stem Cells, 8(2), 47.
Chen, R., Hao, Z., Wang, Y., Zhu, H., Hu, Y., Chen, T., Zhang, P., & Li, J. (2022). Mesenchymal stem cell–immune cell interaction and related modulations for bone tissue engineering. Stem Cells International, 2022.
Chen, Z., Jin, M., He, H., Dong, J., Li, J., Nie, J., Wang, Z., Xu, J., & Wu, F. (2023). Mesenchymal stem cells and macrophages and their interactions in tendon-bone healing. Journal of Orthopaedic Translation, 39, 63–73.
de Los Reyes Jiménez, M., Lechner, A., Alessandrini, F., Bohnacker, S., Schindela, S., Trompette, A., Haimerl, P., Thomas, D., Henkel, F., & Mourão, A. (2020). An anti-inflammatory eicosanoid switch mediates the suppression of type-2 inflammation by helminth larval products. Science Translational Medicine, 12(540), eaay0605.
Driscoll, J., & Patel, T. (2019). The mesenchymal stem cell secretome as an acellular regenerative therapy for liver disease. Journal of Gastroenterology, 54, 763–773.
Hati, I. P., Putra, A., & Sumarawati, T. (2023). Effect of Topical Gel Administration of Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) on IL-10 and TNF-α Gene Expression (In Vivo Experimental Study in Male Rats of Wistar Strains Model of Fluconazole-Induced Alopecia). INTERNATIONAL JOURNAL OF MULTIDISCIPLINARY RESEARCH AND ANALYSIS, 6(12), 5600–5610.
Kandilarov, I., Gardjeva, P., Georgieva-Kotetarova, M., Zlatanova, H., Vilmosh, N., Kostadinova, I., Katsarova, M., Atliev, K., & Dimitrova, S. (2023). Effect of Plant Extracts Combinations on TNF-α, IL-6 and IL-10 Levels in Serum of Rats Exposed to Acute and Chronic Stress. Plants, 12(17), 3049.
Kumar, S., Kabat, M., Basak, S., Babiarz, J., Berthiaume, F., & Grumet, M. (2022). Anti-inflammatory effects of encapsulated human mesenchymal stromal/stem cells and a method to scale-up cell encapsulation. Biomolecules, 12(12), 1803.
Kusuma, Y. T., & Subchan, P. (2023). Effect of Gel Secretome Hypoxia Mesenchymal Stem Cell on Expression of TGF- β and IL-6 (In Vivo Experimental Study in Male Rats of Wistar Strains Model Hyperglycemic Wounds). International Journal of Multidisciplinary Research and Analysis, 06(12). https://doi.org/10.47191/ijmra/v6-i12-23
Landázuri, N., Levit, R. D., Joseph, G., Ortega‐Legaspi, J. M., Flores, C. A., Weiss, D., Sambanis, A., Weber, C. J., Safley, S. A., & Taylor, W. R. (2016). Alginate microencapsulation of human mesenchymal stem cells as a strategy to enhance paracrine‐mediated vascular recovery after hindlimb ischaemia. Journal of Tissue Engineering and Regenerative Medicine, 10(3), 222–232.
Liang, X., Ding, Y., Zhang, Y., Tse, H.-F., & Lian, Q. (2014). Paracrine mechanisms of mesenchymal stem cell-based therapy: current status and perspectives. Cell Transplantation, 23(9), 1045–1059.
Liem, I. K. (2015). Simple production method of umbilical cord derived mesenchymal stem cell using xeno-free materials for translational research. In Journal of Chemical and Pharmaceutical Research (p. 8). JOCPR.
Liu, D., Huang, S.-Y., Sun, J.-H., Zhang, H.-C., Cai, Q.-L., Gao, C., Li, L., Cao, J., Xu, F., & Zhou, Y. (2022). Sepsis-induced immunosuppression: mechanisms, diagnosis and current treatment options. Military Medical Research, 9(1), 1–19.
Markovics, A., Rosenthal, K. S., Mikecz, K., Carambula, R. E., Ciemielewski, J. C., & Zimmerman, D. H. (2021). Restoring the balance between pro-inflammatory and anti-inflammatory cytokines in the treatment of rheumatoid arthritis: New insights from animal models. Biomedicines, 10(1), 44.
Mead, B., Chamling, X., Zack, D. J., Ahmed, Z., & Tomarev, S. (2020). TNFα-mediated priming of mesenchymal stem cells enhances their neuroprotective effect on retinal ganglion cells. Investigative Ophthalmology & Visual Science, 61(2), 6.
Meftahpour, V., Malekghasemi, S., Baghbanzadeh, A., Aghebati-Maleki, A., Pourakbari, R., Fotouhi, A., & Aghebati-Maleki, L. (2021). Platelet lysate: a promising candidate in regenerative medicine. Regenerative Medicine, 16(01), 71–85.
Nurhayati, R. W., Antarianto, R. D., Pratama, G., Rahayu, D., Mubarok, W., Kobayashi, M., & Hutabarat, M. (2019). Encapsulation of human hematopoietic stem cells with a biocompatible polymer. AIP Conference Proceedings, 2092(1).
Porro, C., Cianciulli, A., & Panaro, M. A. (2020). The regulatory role of IL-10 in neurodegenerative diseases. Biomolecules, 10(7), 1017.
Putra, A., Ridwan, F. B., Putridewi, A. I., Kustiyah, A. R., Wirastuti, K., Sadyah, N. A. C., Rosdiana, I., & Munir, D. (2018). The role of TNF-α induced MSCs on suppressive inflammation by increasing TGF-β and IL-10. Open Access Macedonian Journal of Medical Sciences, 6(10), 1779.
Saldaña, L., Bensiamar, F., Vallés, G., Mancebo, F. J., García-Rey, E., & Vilaboa, N. (2019). Immunoregulatory potential of mesenchymal stem cells following activation by macrophage-derived soluble factors. Stem Cell Research & Therapy, 10(1), 1–15.
Sibuea, C. V., Pawitan, J. A., Antarianto, R., Jasirwan, C. O. M., Sianipar, I. R., Luviah, E., Nurhayati, R. W., Mubarok, W., & Mazfufah, N. F. (2020). 3D Co-Culture of Hepatocyte, a Hepatic Stellate Cell Line, and Stem Cells for Developing a Bioartificial Liver Prototype. International Journal of Technology 11(5), 11(5), 95–962.
Tsuji, W., Rubin, J. P., & Marra, K. G. (2014). Adipose-derived stem cells: Implications in tissue regeneration. World Journal of Stem Cells, 6(3), 312.
Ulivi, V., Tasso, R., Cancedda, R., & Descalzi, F. (2014). Mesenchymal stem cell paracrine activity is modulated by platelet lysate: induction of an inflammatory response and secretion of factors maintaining macrophages in a proinflammatory phenotype. Stem Cells and Development, 23(16), 1858–1869.
Wang, J., Chen, Z., Li, M., Song, Y., Xu, W., Wang, L., & Chen, S. (2022). Genome-wide identification, immune response profile and functional characterization of IL-10 from spotted knifejaw (Oplegnathus punctatus) during host defense against bacterial and viral infection. Fish & Shellfish Immunology, 124, 513–524.
Yani, S., & Pawitan, J. A. (2023). Stem cell mechanism of action in neuroplasticity after stroke. Egyptian Pharmaceutical Journal, 22(3), 344–352.
Chen, R., Hao, Z., Wang, Y., Zhu, H., Hu, Y., Chen, T., Zhang, P., & Li, J. (2022). Mesenchymal stem cell–immune cell interaction and related modulations for bone tissue engineering. Stem Cells International, 2022.
Chen, Z., Jin, M., He, H., Dong, J., Li, J., Nie, J., Wang, Z., Xu, J., & Wu, F. (2023). Mesenchymal stem cells and macrophages and their interactions in tendon-bone healing. Journal of Orthopaedic Translation, 39, 63–73.
de Los Reyes Jiménez, M., Lechner, A., Alessandrini, F., Bohnacker, S., Schindela, S., Trompette, A., Haimerl, P., Thomas, D., Henkel, F., & Mourão, A. (2020). An anti-inflammatory eicosanoid switch mediates the suppression of type-2 inflammation by helminth larval products. Science Translational Medicine, 12(540), eaay0605.
Driscoll, J., & Patel, T. (2019). The mesenchymal stem cell secretome as an acellular regenerative therapy for liver disease. Journal of Gastroenterology, 54, 763–773.
Hati, I. P., Putra, A., & Sumarawati, T. (2023). Effect of Topical Gel Administration of Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) on IL-10 and TNF-α Gene Expression (In Vivo Experimental Study in Male Rats of Wistar Strains Model of Fluconazole-Induced Alopecia). INTERNATIONAL JOURNAL OF MULTIDISCIPLINARY RESEARCH AND ANALYSIS, 6(12), 5600–5610.
Kandilarov, I., Gardjeva, P., Georgieva-Kotetarova, M., Zlatanova, H., Vilmosh, N., Kostadinova, I., Katsarova, M., Atliev, K., & Dimitrova, S. (2023). Effect of Plant Extracts Combinations on TNF-α, IL-6 and IL-10 Levels in Serum of Rats Exposed to Acute and Chronic Stress. Plants, 12(17), 3049.
Kumar, S., Kabat, M., Basak, S., Babiarz, J., Berthiaume, F., & Grumet, M. (2022). Anti-inflammatory effects of encapsulated human mesenchymal stromal/stem cells and a method to scale-up cell encapsulation. Biomolecules, 12(12), 1803.
Kusuma, Y. T., & Subchan, P. (2023). Effect of Gel Secretome Hypoxia Mesenchymal Stem Cell on Expression of TGF- β and IL-6 (In Vivo Experimental Study in Male Rats of Wistar Strains Model Hyperglycemic Wounds). International Journal of Multidisciplinary Research and Analysis, 06(12). https://doi.org/10.47191/ijmra/v6-i12-23
Landázuri, N., Levit, R. D., Joseph, G., Ortega‐Legaspi, J. M., Flores, C. A., Weiss, D., Sambanis, A., Weber, C. J., Safley, S. A., & Taylor, W. R. (2016). Alginate microencapsulation of human mesenchymal stem cells as a strategy to enhance paracrine‐mediated vascular recovery after hindlimb ischaemia. Journal of Tissue Engineering and Regenerative Medicine, 10(3), 222–232.
Liang, X., Ding, Y., Zhang, Y., Tse, H.-F., & Lian, Q. (2014). Paracrine mechanisms of mesenchymal stem cell-based therapy: current status and perspectives. Cell Transplantation, 23(9), 1045–1059.
Liem, I. K. (2015). Simple production method of umbilical cord derived mesenchymal stem cell using xeno-free materials for translational research. In Journal of Chemical and Pharmaceutical Research (p. 8). JOCPR.
Liu, D., Huang, S.-Y., Sun, J.-H., Zhang, H.-C., Cai, Q.-L., Gao, C., Li, L., Cao, J., Xu, F., & Zhou, Y. (2022). Sepsis-induced immunosuppression: mechanisms, diagnosis and current treatment options. Military Medical Research, 9(1), 1–19.
Markovics, A., Rosenthal, K. S., Mikecz, K., Carambula, R. E., Ciemielewski, J. C., & Zimmerman, D. H. (2021). Restoring the balance between pro-inflammatory and anti-inflammatory cytokines in the treatment of rheumatoid arthritis: New insights from animal models. Biomedicines, 10(1), 44.
Mead, B., Chamling, X., Zack, D. J., Ahmed, Z., & Tomarev, S. (2020). TNFα-mediated priming of mesenchymal stem cells enhances their neuroprotective effect on retinal ganglion cells. Investigative Ophthalmology & Visual Science, 61(2), 6.
Meftahpour, V., Malekghasemi, S., Baghbanzadeh, A., Aghebati-Maleki, A., Pourakbari, R., Fotouhi, A., & Aghebati-Maleki, L. (2021). Platelet lysate: a promising candidate in regenerative medicine. Regenerative Medicine, 16(01), 71–85.
Nurhayati, R. W., Antarianto, R. D., Pratama, G., Rahayu, D., Mubarok, W., Kobayashi, M., & Hutabarat, M. (2019). Encapsulation of human hematopoietic stem cells with a biocompatible polymer. AIP Conference Proceedings, 2092(1).
Porro, C., Cianciulli, A., & Panaro, M. A. (2020). The regulatory role of IL-10 in neurodegenerative diseases. Biomolecules, 10(7), 1017.
Putra, A., Ridwan, F. B., Putridewi, A. I., Kustiyah, A. R., Wirastuti, K., Sadyah, N. A. C., Rosdiana, I., & Munir, D. (2018). The role of TNF-α induced MSCs on suppressive inflammation by increasing TGF-β and IL-10. Open Access Macedonian Journal of Medical Sciences, 6(10), 1779.
Saldaña, L., Bensiamar, F., Vallés, G., Mancebo, F. J., García-Rey, E., & Vilaboa, N. (2019). Immunoregulatory potential of mesenchymal stem cells following activation by macrophage-derived soluble factors. Stem Cell Research & Therapy, 10(1), 1–15.
Sibuea, C. V., Pawitan, J. A., Antarianto, R., Jasirwan, C. O. M., Sianipar, I. R., Luviah, E., Nurhayati, R. W., Mubarok, W., & Mazfufah, N. F. (2020). 3D Co-Culture of Hepatocyte, a Hepatic Stellate Cell Line, and Stem Cells for Developing a Bioartificial Liver Prototype. International Journal of Technology 11(5), 11(5), 95–962.
Tsuji, W., Rubin, J. P., & Marra, K. G. (2014). Adipose-derived stem cells: Implications in tissue regeneration. World Journal of Stem Cells, 6(3), 312.
Ulivi, V., Tasso, R., Cancedda, R., & Descalzi, F. (2014). Mesenchymal stem cell paracrine activity is modulated by platelet lysate: induction of an inflammatory response and secretion of factors maintaining macrophages in a proinflammatory phenotype. Stem Cells and Development, 23(16), 1858–1869.
Wang, J., Chen, Z., Li, M., Song, Y., Xu, W., Wang, L., & Chen, S. (2022). Genome-wide identification, immune response profile and functional characterization of IL-10 from spotted knifejaw (Oplegnathus punctatus) during host defense against bacterial and viral infection. Fish & Shellfish Immunology, 124, 513–524.
Yani, S., & Pawitan, J. A. (2023). Stem cell mechanism of action in neuroplasticity after stroke. Egyptian Pharmaceutical Journal, 22(3), 344–352.