Effect of Vitamin E Against Levels Malondyaldehyde (MDA) and Estrogen Hormone Levels Rats (Rattus Norvegicus) Females Who Exposed to Cigarette Smoke.
Keywords:
Vitamin E, Malondyaldehyde (MDA), EstrogenAbstract
Indonesia is one of the countries with the greatest prevalence of smokers in the world. Exposure to cigarette smoke can lead to the physiological impact for women that can inhibit estrogen synthesis in the ovaries and increase levels of Malondyaldehyde (MDA). The purpose of this study to analyze the effect of vitamin E against MDA and estrogen rat (Rattus norvegicus) females were exposed to smoke. This research is an experimental design Post-Test Only Control Group. The total sample consisted of 30 rats females were divided into 5 groups: negative control group without treatment, the positive control group were given exposure to cigarette smoke 1 cigarettes / day and 3 treatment groups P1, P2 and P3 with vitamin E doses of multilevel: 100, 200 and 400 mg / kg / day and exposure to tobacco smoke 1 cigarettes / day. Research carried out for 21 days at Animal house and in the Biomedical Laboratory Faculty of Medicine, University of Andalas Padang. MDA and estrogen hormone levels were measured using ELISA method. Test of significance with One Way ANOVA followed by Bonferroni Multiple Comparisons types. The results showed significant differences (p <0.05) MDA levels between the negative control group (0.53 ± 0.04) positive control group (0.67 ± 0.07). In the estrogen levels also differed significantly (p <0.05) between the negative control group (0.27 ± 0.04) with P2 group (0.38 ± 0.07). In conclusion, there is no effect of vitamin E against MDA both in the negative control group and positive control group.
Downloads
References
Abdoulhossein, D., Taheri, I., Saba, M. ali, Akbari, H., Shafagh, S., & Zataollah, A. (2018). Effect of vitamin C and vitamin E on lung contusion: A randomized clinical trial study. Annals of Medicine and Surgery, 36, 152–157. doi:10.1016/j.amsu.2018.10.026
Banjarnahor, S. (2018). The Correlation between Chemotherapy (Vesikan) and Extravasation in Cancer Patients in Teguh Pure Hospital in 2018. Science Midwifery, 7(1, October), 1-10. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/11
Sarah, M. (2018). Effect of Hook/Knitting Activities Against Decreased Stress Levels in Class II-A Medan Women’s Laps. Science Midwifery, 7(1, October), 11-14. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/12
Fatmawati, D., Ismawati, I., & Suriawanto, N. (2018). Factors That Influence the Documentation of the Completeness of Nursing Care by the Implementing Nurse in the Emergency Room at Anutapura General Hospital, Palu. Science Midwifery, 7(1, October), 15-21. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/13
Sunarti, N., & Patimah, I. (2018). Relationship between Knowledge Level and Efforts to Control Blood Pressure in Hypertension Patients in the Work Area of Karangmulya Health Center, Karangpawitan District, Garut Regency. Science Midwifery, 7(1, October), 22-25. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/14
Prautami, E. S. (2018). Knowledge and Attitudes of Pregnant Women during Antenatal Care Visit at Abi Ummi DW Sarmadi Clinic Palembang Relationship Knowledge and Attitude of Pregnant Women Against Antenatal Care Visit In Abi Ummi DW Sarmadi Clinic Palembang. Science Midwifery, 7(1, October), 26-31. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/16
Febrianti, A. (2018). The Relationship between Socio-Economic Factors, Mother’s Knowledge of Healthy Environment and Diarrhea with the Incidence of Diarrhea in Toddlers Aged 1-5 Years at Puskesmas Pembina Palembang . Science Midwifery, 7(1, October), 32-37. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/17
Hastuti, H., Januarista, A., & Suriawanto, N. (2018). Relationship between Self Care and Quality of Life for Pregnant Women with Diabetes Mellitus Patients . Science Midwifery, 7(1, October), 38-44. Retrieved from http://midwifery.iocspublisher.org/index.php/midwifery/article/view/18
Alonso, R., Ruiz, M., Lovera, R., Montes De Oca, D. P., Cavia, R., & Sánchez, J. P. (2019). Norway rat (Rattus norvegicus) ectoparasites in livestock production systems from central Argentina: influencing factors on parasitism. Acta Tropica, 105299. doi:10.1016/j.actatropica.2019.105299
Applegate, J. R., Troan, B. V., Chen, L. R., & Suter, S. E. (2017). Surgical Management of Colonic Adenocarcinoma in a Rat (Rattus norvegicus). Journal of Exotic Pet Medicine, 26(1), 47–52. doi:10.1053/j.jepm.2016.10.011
Asbaghi, O., Choghakhori, R., & Abbasnezhad, A. (2019). Effect of Omega-3 and vitamin E co-supplementation on serum lipids concentrations in overweight patients with metabolic disorders: A systematic review and meta-analysis of randomized controlled trials. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 13(4), 2525–2531. doi:10.1016/j.dsx.2019.07.00
Ataei-Almanghadim, K., Farshbaf-Khalili, A., Ostadrahimi, A. R., Shaseb, E., & Mirghafourvand, M. (2019). The effect of oral capsule of curcumin and vitamin E on the hot flashes and anxiety in postmenopausal women: a triple blind randomised controlled trial. Complementary Therapies in Medicine, 102267. doi:10.1016/j.ctim.2019.102267
Ayala, A. Munoz, MF. Argiieles, S. Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonanal. Oxidative Medicine and Cellular Longevity. 2014: article ID 360438: 31.
Beshay, VE. & Carr, BR. Hypothalamic-Pituitary-Ovarian Axis and Control of the Menstrual Cycle. University of Texas: 2013.
Boros, ÃÂÂ., Orlovácz, K., Pankovics, P., Szekeres, S., Földvári, G., Fahsbender, E., … Reuter, G. (2019). Diverse picornaviruses are prevalent among free-living and laboratory rats (Rattus norvegicus) in Hungary and can cause disseminated infections. Infection, Genetics and Evolution, 103988. doi:10.1016/j.meegid.2019.103988
Colombo, ML. An Update on Vitamin E, Tocopherol and Tocotrienol-Perspective. Journal Molecules. 2010; 15:2103-2113.
Correa, J. P., Bucarey, S. A., Cattan, P. E., Landaeta-Aqueveque, C., & RamÃÂÂrez-Estrada, J. (2017). Renal carriage of Leptospira species in rodents from Mediterranean Chile: The Norway rat ( Rattus norvegicus ) as a relevant host in agricultural lands. Acta Tropica, 176, 105–108. doi:10.1016/j.actatropica.2017.07.032
Duran, M. Aydin, K. Oner, G. Nermin, K. Sarac, GN. Turhan, N. Possible Protective Effect of Applying Citamin E to the Rats Having Been Exposed to Cigarette Smoke: An Animal Study. Global Advanced Research Journals. 2015; 4(4):183-189.
Frankova, M., Kaftanova, B., Aulicky, R., Rodl, P., Frynta, D., & Stejskal, V. (2019). Temporal production of coloured faeces in wild roof rats (Rattus rattus) following consumption of fluorescent non-toxic bait and a comparison with wild R. norvegicus and Mus musculus. Journal of Stored Products Research, 81, 7–10. doi:10.1016/j.jspr.2018.12.002
Galano, A. Free Radicals Induced Oxidative Stress at a Molecular Level: The Current Status, Challenges and Perspectives of Computational Chemistry Based Protocols. Journal Mexico Chem. Soc. 2015; 59(4): 231-262.
Galán-Puchades, M. T., Sanxis-Furió, J., Pascual, J., Bueno-MarÃÂÂ, R., Franco, S., Peracho, V., … Fuentes, M. V. (2018). First survey on zoonotic helminthosis in urban brown rats (Rattus norvegicus) in Spain and associated public health considerations. Veterinary Parasitology, 259, 49–52. doi:10.1016/j.vetpar.2018.06.023
Halliwell, B. The Wanderings of a Free Radical. Journal of Elsevier. 2009.
Idris, R. Hartamto, H. Pengaruh Asap Rokok Kretek Terhadap Imunitas Seluler Tikus Betina Strain LMR. Jurnal Keperawatan Indonesia. 2006;10(2); 41-47.
Jaggi, S. Yadav, AS. Increased Serum Malondialdehyde Levels Among Cigarette Smokers. The Pharma Innovation Journal. 2015; 4(4):94-96.
Kasture, V., Kale, A., Randhir, K., Sundrani, D., & Joshi, S. (2019). Effect of maternal omega-3 fatty acids and vitamin E supplementation on placental apoptotic markers in rat model of early and late onset preeclampsia. Life Sciences, 117038. doi:10.1016/j.lfs.2019.117038
Koizumi, N., Miura, K., Sanai, Y., Takemura, T., Hong Trang, U. T., Thanh, L. T., … Ohnishi, M. (2019). Molecular epidemiology of Leptospira interrogans in Rattus norvegicus in Hanoi, Vietnam. Acta Tropica. doi:10.1016/j.actatropica.2019.02.008
Marroquin, S. C., Eshar, D., Browning, G. R., & Herrin, B. (2019). DIAGNOSIS AND SUCCESSFUL TREATMENT OF EIMERIA INFECTION IN A PAIR OF PET DOMESTIC RATS (RATTUS NORVEGICUS) WITH PONAZURIL. Journal of Exotic Pet Medicine. doi:10.1053/j.jepm.2019.02.002
Mirzavandi, F., Talenezhad, N., Razmpoosh, E., Nadjarzadeh, A., & Mozaffari-Khosravi, H. (2020). The effect of intramuscular megadose of vitamin D injections on E-selectin, CRP and biochemical parameters in vitamin D-deficient patients with type-2 diabetes mellitus: A randomized controlled trial. Complementary Therapies in Medicine, 49, 102346. doi:10.1016/j.ctim.2020.102346
Pichandi, S. Pasupathi, P. Rao, YY. Farook, J., Ponnusha, BS. Ambika, A. et al. The Effect of Smoking on Canser- A review. Intarnational Journal of Biological & Medical Reasearch. 2011; 2(2): 593-602.
Pitargue, F. M., Kim, J. H., Goo, D., Delos Reyes, J. B., & Kil, D. Y. (2019). Effect of vitamin E sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens. Poultry Science. doi:10.3382/ps/pez149
Pitargue, F. M., Kim, J. H., Goo, D., Delos Reyes, J. B., & Kil, D. Y. (2019). Effect of vitamin E sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens. Poultry Science. doi:10.3382/ps/pez149
Rahmawati, RSN. Saputri, IK. Ratnawati, R. Soeharto, S. Wiyasa, WA. The Effect of α-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes. Cukurova Medical Journal. 2014; 39 (2): 203-212.
Rizvi, S. Raza, ST. Ahmed, F. Ahmad, A. Abbas, S. Mahdi, F. The Role of Vitamin E in Human Health and Some Disease. Sultan Qaboos University Journal. 2013; 14(2):157-165.
Ryan, MJ. Dudash, HJ. Docherty, M. Geronilla, KB. Baker, BA. Haff, GG. et al. Vitamin E and C Supplementation Reduces Oxidative Stress, Improves Antioxidant Enzymes and Positive Muscle Work in Chronically Loaded Muscles of Aged Rats. Experimental Gerontology. 2010;45(11): 882-895.
Sepidarkish, M., Akbari-Fakhrabadi, M., Daneshzad, E., Yavari, M., Rezaeinejad, M., Morvaridzadeh, M., & Heshmati, J. (2019). Effect of omega-3 fatty acid plus vitamin E Co-Supplementation on oxidative stress parameters: a systematic review and meta-analysis. Clinical Nutrition. doi:10.1016/j.clnu.2019.05.004
Sepidarkish, M., Morvaridzadeh, M., Akbari-Fakhrabadi, M., Almasi-Hashiani, A., Rezaeinejad, M., & Heshmati, J. (2019). Effect of omega-3 fatty acid plus vitamin E Co-Supplementation on lipid profile: A systematic review and meta-analysis. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. doi:10.1016/j.dsx.2019.03.018
Soler, V., Cuvertoret-Sanz, M., Ramis, A., & Martorell, J. (2019). Subcutaneous lymphangiosarcoma in a pet rat. Journal of Exotic Pet Medicine. doi:10.1053/j.jepm.2019.06.003
Tabassum, B., Bajaj, P., Fathi Abd_Allah, E., Hashem, A., & Hashim, M. (2018). Bioremediation of Cadmium induced renal toxicity in Rattus norvegicus by medicinal plant Catharanthus roseus. Saudi Journal of Biological Sciences. doi:10.1016/j.sjbs.2018.09.009
Tariq, N., Basharat, Z., Butt, S., & Baig, D. N. (2016). Distribution analysis of profilin isoforms at transcript resolution with mRNA-seq and secondary structure in various organs of Rattus norvegicus. Gene, 589(1), 49–55. doi:10.1016/j.gene.2016.05.023
Wang, L., Ma, B., Chen, D., Lou, B., Zhan, W., Chen, R., … Xie, Q. (2019). Effect of dietary level of vitamin E on growth performance, antioxidant ability, and resistance to Vibrio alginolyticus challenge in yellow drum Nibea albiflora. Aquaculture, 507, 119–125. doi:10.1016/j.aquaculture.2019.04.003
Zorawar, S. Indrakaran, P. Karthigesu. Pramjit, S. Rupinder, K. Use of Malondialdehyde as a Biomarker for Assesing Oxidative Stress in Different Disease Pathologies: a Review. Iranian Journal Public Health. 2014; 43.(3): 7-16.
