Isolation and identification of escherichia coli in well water located in Garut Regency
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Apr 29, 2023
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Abstract
Water is one of the most important needs for daily life, especially well water. In the Garut district area well water is used for daily activities ranging from washing, drinking, bathing, ablution to being used for drinking water consumption. This study aims to isolate and identify well water from 10 villages located in the Garut Regency. Sampling was done using purposive sampling method. The test was followed by a presumptive test using Lactose Broth (LB), a reinforcing test using 2% BGLB media and a complementary test consisting of EMB agar media and biochemical tests. The results showed that of the 10 well water samples, 7 met the requirements of proper water quality and 3 did not meet the requirements of clean water with MPN values from three villages, namely Kampung Tanjung kamuning 200/ml, Kampung salaawi 241/100ml and Kampung Cipepe 241/100ml. EMB agar media is characterized by the formation of a metallic green color with black spots colonies and biochemical tests that show the well water has been contaminated with Escherichia coli bacteria.
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How to Cite
Sugiah, S., Mutmaina, G. N., Mamay, M. and Nurisani, A. (2023) “Isolation and identification of escherichia coli in well water located in Garut Regency”, Science Midwifery, 11(1), pp. 195-201. doi: 10.35335/midwifery.v11i1.1236.
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Hajare, S. T., Chekol, Y., & Chauhan, N. M. (2022). Assessment of prevalence of Giardia lamblia infection and its associated factors among government elementary school children from Sidama zone, SNNPR …. In Plos one. journals.plos.org. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0264812
Haribi, R., & Yusron, K. (2010). Pemeriksaan Escherichia coli pada air bak Wudhlu 10 Masjid di Kecamatan Tlogosari Semarang. Jurnal Kesehatan Unimus, 3(1), 105647.
Hikmah, N., Dewi, A., Shita, P., & Maulana, H. (2015). Rat Diabetic Blood Glucose Level Profile with Stratified Dose Streptozotocin ( SD-STZ ) and Multi Low Dose Streptozotocin ( MLD-STZ ) Induction Methods. The Journal of Tropical Life Science, 5(1), 30–34.
Huang, M. T. (2021). Achieving Paris Agreement temperature goals requires carbon neutrality by middle century with far-reaching transitions in the whole society. Advances in Climate Change Research, 12(2), 281–286. https://doi.org/10.1016/j.accre.2021.03.004
Husna, F., Suyatna, F. D., Arozal, W., & Purwaningsih, E. H. (2019). Model Hewan Coba pada Penelitian Diabetes. Pharmaceutical Sciences and Research (PSR), 6(3), 131–141.
Jamilatun, M., & Aminah, A. (2016). Isolasi Dan Identifikasi Escherichia Coli Pada Air Wudhu Di Masjid Yang Berada Di Kota Tangerang. Jurnal Medikes (Media Informasi Kesehatan), 3(1), 81–90. https://doi.org/10.36743/medikes.v3i1.154
Jasper, E. E. (2020). Nonlinear regression analysis of the sorption of crystal violet and methylene blue from aqueous solutions onto an agro-waste derived activated carbon. Applied Water Science, 10(6). https://doi.org/10.1007/s13201-020-01218-y
Pratama, B. A., Susanto, A., & Aini, I. (2020). Identifikasi Bakteri Escherichia Coli Pada Air SUmur Gali Di Desa Gempollegundi Kecamatan Gudo. In STIKes Insan Cendekia Medika Jombang (Vol. 1, Issue 1).
Putri, D. D. N., & Suriadi. (2015). Pengaruh Daun Insulin (Smallanthus sonchifolius) Terhadap Penurunan Kadar Glukosa Darah pada Tikus Diabetes Melitus. Jurnal Proners, 3(1). https://doi.org/http://dx.doi.org/10.26418/jpn.v3i1.21329
Saputra, N. T., Suartha, N. I., & Dharmayudha, A. A. G. O. (2018). Agen Diabetagonik Streptozotocin untuk Membuat Tikus Putih Jantan Diabetes Mellitus. Buletin Veteriner Udayana, 10(2), 116–121. https://doi.org/10.24843/bulvet.2018.v10.i02.p02
Siti Nurkomala Sari, Ety Apriliana, Susianti, T. U. S. (2019). Identifikasi Bakteri BABI. Medula, 9(1), 57–65.
Soltani, N., Keshavarz, M., & Dehpour, A. R. (2007). Effect of oral magnesium sulfate administration on blood pressure and lipid profile in streptozocin diabetic rat. European Journal of Pharmacology, 560(2–3), 201–205. https://doi.org/10.1016/j.ejphar.2006.12.020