The impact of using Binahong (Anredera Cardifolia) leaf extract on male wistar white rats fed a high-fat diet's liver function and liver histopathology


Fitria Marizka Kusumawardhany
Hanjaya Hanjaya
Buter Samin


Worldwide, Nonalcoholic Fatty Liver Disease (NAFLD) is the leading cause of chronic liver disease. Nutritional excess causes adipose depot enlargement and ectopic fat buildup, causing NAFLD. The Binahong plant (Anredera cordifolia) contains saponins, alkaloids, polyphenols, flavonoids, and mono polysaccharides like L-arabinose, D-galactose, L-rhamnose, and D-glucose, which have been shown to help NAFLD. The study examined the liver function and histopathology of male white Wistar rats fed high-fat diets after Binahong leaf extract was administered. Research approach/sample/population: Quantitative research employing lab or experiment formats. We sampled 24 white Wistar rats (Rattus norvegicus). A high-fat diet was the precondition, Binahong leaf extract was the independent variable, and liver function and histological characteristics improved. The research approach begins with test animal acclimation, Binahong leaf extract extraction, phytochemical screening, treatment protocols, examination, and Histopathology preparations. Research data was processed with SPSS. The normality test shows a 2-tailed significance of 0.678 > 0.05, indicating regularly distributed data. SGOT 0.10 and SGPT 0.20 liver function analyses demonstrated homogeneity p values > 0.05. The research found that Binahong leaf extract (Anrederacordifolia Ten. Steenis) has 400mg/BW antioxidants and can restore liver function in high-fat livers.



How to Cite
Kusumawardhany, F. M., Hanjaya, H. and Samin, B. (2024) “The impact of using Binahong (Anredera Cardifolia) leaf extract on male wistar white rats fed a high-fat diet’s liver function and liver histopathology”, Science Midwifery, 12(2), pp. 686-695. doi: 10.35335/midwifery.v12i2.1528.


Anderson, E. L., Howe, L. D., Fraser, A., Macdonald-Wallis, C., Callaway, M. P., Sattar, N., Day, C., Tilling, K., & Lawlor, D. A. (2015). Childhood Energy Intake Is Associated with Nonalcoholic Fatty Liver Disease in Adolescents. The Journal of Nutrition, 145(5), 983–989.
Bedossa, P. (2017). Pathology of non‐alcoholic fatty liver disease. Liver International, 37(S1), 85–89.
Bellentani, S. (2017). The epidemiology of non‐alcoholic fatty liver disease. Liver International, 37(S1), 81–84.
Berumen, J., Baglieri, J., Kisseleva, T., & Mekeel, K. (2021). Liver fibrosis: Pathophysiology and clinical implications. WIREs Mechanisms of Disease, 13(1).
Cariello, R., Federico, A., Sapone, A., Tuccillo, C., Scialdone, V. R., Tiso, A., Miranda, A., Portincasa, P., Carbonara, V., Palasciano, G., Martorelli, L., Esposito, P., Cartenì, M., Del Vecchio Blanco, C., & Loguercio, C. (2010). Intestinal permeability in patients with chronic liver diseases: Its relationship with the aetiology and the entity of liver damage. Digestive and Liver Disease, 42(3), 200–204.
Cena, H., & Calder, P. C. (2020). Defining a healthy diet: Evidence for the role of contemporary dietary patterns in health and disease. Nutrients, 12(2).
Djamil, R., Winarti, W., Zaidan, S., & Abdillah, S. (2017). Antidiabetic Activity of Flavonoid from Binahong Leaves (Anredera cordifolia) Extract in Alloxan Induced Mice. Journal of Pharmacognosy & Natural Products, 03(02).
Duke, J. A., Bogenschutz-Godwin, M. J., DuCellier, J., Duke, P.-A. K., & Kumar, R. (2022). Handbook of Medicinal Herbs Second Edition (Kindle Edi, Vol. 5, Issue 1). Florida: CRC Press.
Francque, S. M., Marchesini, G., Kautz, A., Walmsley, M., Dorner, R., Lazarus, J. V., Zelber-Sagi, S., Hallsworth, K., Busetto, L., Frühbeck, G., Dicker, D., Woodward, E., Korenjak, M., Willemse, J., Koek, G. H., Vinker, S., Ungan, M., Mendive, J. M., & Lionis, C. (2021). Non-alcoholic fatty liver disease: A patient guideline. JHEP Reports, 3(5), 100322.
Ghozali, I. (2018). Aplikasi Analisis Multivariate dengan Program IBM SPSS 25. In Badan Penerbit Universitas Diponegoro.
Hallsworth, K., & Adams, L. A. (2019). Lifestyle modification in NAFLD/NASH: Facts and figures. JHEP Reports, 1(6), 468–479.
Koutoukidis, D. A., Koshiaris, C., Henry, J. A., Noreik, M., Morris, E., Manoharan, I., Tudor, K., Bodenham, E., Dunnigan, A., Jebb, S. A., & Aveyard, P. (2021). The effect of the magnitude of weight loss on non-alcoholic fatty liver disease: A systematic review and meta-analysis. Metabolism, 115, 154455.
López-Gil, J. F., & Tárraga-López, P. J. (2022). Research on Diet and Human Health. International Journal of Environmental Research and Public Health, 19(11), 6526.
Moriles, K. E., & Azer, S. A. (2020). Alanine Amino Transferase. StatPearls.
Murel, P. (2017). Liver Pathophysiology: Therapies & Antioxidants. In P. Murel (Ed.), Liver Pathophysiology: Therapies & Antioxidants (1st ed.). Academic Press.
Neuschwander-Tetri, B. A. (2017). Non-alcoholic fatty liver disease. BMC Medicine, 15(1), 45.
Notoatmodjo, S. (2022). Metodologi Penelitian Kesehatan (3rd ed.). Jakarta: Rineka Cipta.
Ojutalayo Adeeyo, A., Mellda Ndou, T., Adewumi Alabi, M., Dumisani Mkoyi, H., Motunrayo Enitan, E., Beswa, D., Makungo, R., & O. Odiyo, J. (2021). Structure: Activity and Emerging Applications of Spices and Herbs. In Herbs and Spices - New Processing Technologies. IntechOpen.
Ozougwu, J. C. (2017). Physiology of the liver. International Journal of Research in Pharmacy and Biosciences, 14(8), 13–24.
Parola, M., & Pinzani, M. (2019). Liver fibrosis: Pathophysiology, pathogenetic targets and clinical issues. Molecular Aspects of Medicine, 65, 37–55.
Pinzani, M., Rombouts, K., & Colagrande, S. (2005). Fibrosis in chronic liver diseases: diagnosis and management. Journal of Hepatology, 42(1), S22–S36.
Powell, E. E., Wong, V. W.-S., & Rinella, M. (2021). Non-alcoholic fatty liver disease. The Lancet, 397(10290), 2212–2224.
Roehlen, N., Crouchet, E., & Baumert, T. F. (2020). Liver Fibrosis: Mechanistic Concepts and Therapeutic Perspectives. Cells, 9(4), 875.
Ross, E. A., Devitt, A., & Johnson, J. R. (2021). Macrophages: The Good, the Bad, and the Gluttony. Frontiers in Immunology, 12.
Sharma, A., & Nagalli, S. (2022). Chronic liver disease. StatPearls Publishing.
Suwarno, B., & Nugroho, A. (2023). Kumpulan Variabel-Variabel Penelitian Manajemen Pemasaran (Definisi & Artikel Publikasi) (1st ed.). Bogor: Halaman Moeka Publishing.
Upadhyay, J., Tiwari, N., Durgapal, S., & Farzaei, M. H. (2022). Chapter5.10 - Antioxidants and liver diseases (S. M. Nabavi & A. S. B. T.-A. E. in H. Silva (eds.); pp. 799–816). Elsevier.
Von Braun, J., Afsana, K., Fresco, L. O., & Hassan, M. H. A. (2023). Science and Innovations for Food Systems Transformation. Springer International Publishing.
Yip, T. C., Wong, V. W., & Wong, G. L. (2021). Alanine Aminotransferase Level: The Road to Normal in 2021. Hepatology Communications, 5(11), 1807–1809.