Antioxidant Activity of Ethanol and Methanol Extracts of Black Turmeric (Curcuma Caesia Roxb.) Using the DPPH (2,2-Diphenyl-1-Picrylhidrazyl) Method

##plugins.themes.academic_pro.article.sidebar##

Published Dec 24, 2022
https://doi.org/10.35335/midwifery.v10i5.990
Download
PDF
Statistic

Downloads

Download data is not yet available.
Vol. 10 No. 5 (2022): December: Science Midwifery

##plugins.themes.academic_pro.article.main##

Ni nyoman wahyu udayani
Universitas Mahasaraswati Denpasar
Ketut Agus Adrianta
Universitas Mahasaraswati Denpasar
IGAA Kusuma Wardani
Universitas Mahasaraswati Denpasar

Abstract

Free radicals are highly reactive compounds in the body that can cause degenerative diseases. One mechanism that can counteract free radicals is antioxidants. Natural antioxidants can be obtained from traditional drinks made from plants that contain active antioxidant compounds such as black turmeric (Curcuma caesia Roxb.) from the Zingiberaceae family. This study aims to determine the antioxidant activity of black turmeric extract. Before testing the antioxidant activity, qualitative and quantitative analysis were carried out. Qualitative analysis was carried out by phytochemical screening. The antioxidant activity of black turmeric extract was tested using the DPPH method. This test begins with the manufacture of extracts and preparation of test samples, then measurement of free radical scavenging activity using a UV-Vis spectrophotometer is carried out. Based on the results of the percentage measurement, the linear regression equation for the ethanol extract was obtained, namely y = 0.1526x + 0.9105 and the value of R2 = 0.9965, and for the methanol extract, namely y = 0.2376x + 1.6809 and the value of R2 = 0.996. According to the linear regression equation, black turmeric extract has antioxidant activity, which is categorized as a very weak antioxidant with an IC50 of 321,687 ppm for ethanol extract and 203, 363 ppm for methanol extract.

##plugins.themes.academic_pro.article.details##

How to Cite
wahyu udayani, N. nyoman, Ketut Agus Adrianta and IGAA Kusuma Wardani (2022) “Antioxidant Activity of Ethanol and Methanol Extracts of Black Turmeric (Curcuma Caesia Roxb.) Using the DPPH (2,2-Diphenyl-1-Picrylhidrazyl) Method”, Science Midwifery, 10(5), pp. 4342-4349. doi: 10.35335/midwifery.v10i5.990.

References

1. Das S, Mondal P ZK. Curcuma caesia Roxb and its medicinal uses: A Review. Int J Res Pharm Chem. 2013;3(2):370–5.
2. Verma, R.K., G. Mishra, P. Singh, K.K. Jha dan RLK. Alpinia Galanga–An Important Medicinal Plant, A Review. Der. Pharmacia Sinica,. Chem J. 2011;2:142–54.
3. Tilaar, Martha dan Widjaja BT. The Power of Jamu. Jakarta: PT Gramedia Pustaka Utama; 2014.
4. Fong YM. In Vitro Regeneration of Kunyit Hitam. 2012;
5. Zuraidah, Sulistiyani DS dan IHS. Fenol, Flavonoid, dan Aktivitas Antioksidan Pada Ekstrak Kulit Batang Pulai (Alstonia scholaris R.Br). J Penelit Has Hutan. 2015;35 No 3.
6. Melannisa, Rosita. Muhammad Da’I RTR. Uji Aktivitas Penangkap Radikal Bebas dan Penetapan Kadar Fenolik Total Ekstrak Etanol Tiga RIMPANG Genus Curcuma Dan Rimpang Temu Kunci (Boesenbergia pandurata). Pharmacon. 2011;12(1):40–3.
7. Munawarah, S. & Handayani PA. Ekstraksi Minyak Daun Jeruk Purut (Cytrus hydtrik D.C) Dengan Pelarut Etanol dan N-Heksan. Kompetensi Tek. 2010;2(1):73–8.
8. RI B. Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor Hk. 03.1.23.06.11.5629. 2011;
9. Amin MR. Analisis Kuantitatif Kadar Flavonoid Ekstrak Etanol Daun Sirsak (Annona Muricata L.) Dengan Metode Spektrofotometri UV-Visible. 2017;
10. Noer, S., Pratiwi, R. D., & Gresinta E. Penetapan Kadar Senyawa Fitokimia (Tanin, Saponin dan Flavonoid) sebagai Kuersetin Pada Ekstrak Daun Inggu (Ruta angustifolia L.). J Eksakta. 2018;18(1):19–29.
11. Ningrum, R., Purwanti, E. & S. Identifikasi Senyawa Alkaloid dari Batang Karamunting (Rhodomyrtus tomentosa) Sebagai Bahan Ajar Biologi Untuk SMA Kelas X. J Pendidik Biol Indinesia. 2016;2(3):231–236.
12. Widi, R. K., & Indriati T. Penjaringan dan Identifikasi Senyawa Alkaloid dalam Batang Kayu Kuning (Arcangelisia Flava Merr). J ILMU DASAR. 2007;8(1):24–9.
13. Tutik RP dan SM. Validasi Metode Analisis Siklamat Secara Spektrofotometri dan Turbidmetri. JSains Dasar. 2015;4(1):23–9.
14. Verma, R.K., G. Mishra, P. Singh, K.K. Jha dan RLK. Alpinia Galanga–An Important Medicinal Plant, A Review. Der. Pharmacia Sinica,. J Chem. 2011;2:142–54.
15. Javanmardi, J., C. Stushnoff, E. Locke and JMV. Antioxidant Activity and Total Phenolic Content of Iranian Ocimum Accessions. Food Chem. 2013;547–50.
16. Widyaningsih W. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Dewa (Gynura procumbens) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil). 2010;
17. Yoga IKW. Penentuan Konsentrasi Optimum Kurva Standar Antioksidan; Asam Galat, Asam Askorbat dan Trolox® terhadap Radikal Bebas DPPH (2,2-diphenyl-1- picrylhydrazyl) 0,1 mM. 2015;
18. Akhlaghi M BB. Review article: mechanisms of flavonoid protection against myocardial ischemia– reperfusion injury. J Mol Cell Cardiol. 2009;46:309–317.
19. Jin YKJ, Russell RM AG. Antioxidant activity and oxidative stress: an overview. Aldini G, Yeum KJ, Niki E RR, editor. Blackwell; 2010.
20. Pinzaru IA, Hădărugă DI, Hădărugă NG, Corpaş L, Grozescu I PF. Hepatoprotective flavonoid b i o c o n j u g a t e / β - c y c l o d e x t r i n nanoparticles:dsc-molecular modeling correlation. Dig J Nanomater Biostructure. 2011;6(4):1605–17.