The immunomodulatory activity of dioscorea alata on spleen histology of rats induced with 50% alcohol
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Mar 25, 2025
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Abstract
Dioscorea alata is frequently consumed and has several health advantages. The immune system-protecting spleen may be protected by bioactive substances in Dioscorea alata tubers. This study examined the influence of immunomodulatory ethanol extract of Dioscorea alata (EEDA) on the diameter of the white pulp of the spleen in 50% alcohol-induced rats (Rattus norvegicus). This research is quasi-experimental using post-test only controlled group design. Twenty-five Rattus norvegicus were separated into five groups. The normal control group (C) received Carboxymextyl Cellulose (CMC), the negative control group (NC) received CMC and 50% alcohol, and three groups received EEDA at doses of 50, 250, and 500 mg/kg for 14 days and 50% alcohol. The diameter of the white pulp of the spleen data analyzed with Kruskal-Wallis and Mann-Whitney tests. The study found a significantly increase in the diameter of the white pulp of the spleen (p<0.05) in the negative control group, EEDA doses of 50 and 250 mg/kg. The control group and EEDA doses 500 mg/kg groups had smaller the diameter of the white pulp of the spleen. The conclusions of this research proved that EEDA has an immunomodulatory activity, EEDA was immunostimulant at doses 50 and 250 mg/kg and immunosuppressant at doses 500 mg/kg.
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How to Cite
Makiyah, S. N. N. and Zahra, A. N. (2025) “The immunomodulatory activity of dioscorea alata on spleen histology of rats induced with 50% alcohol”, Science Midwifery, 13(1), pp. 40-46. doi: 10.35335/midwifery.v13i1.1865.
References
Badan Pusat Statistik. (2023). https://www.bps.go.id/indicator/30/1475/1/konsumsi-alkohol-oleh-penduduk-umur-15-tahun-dalam-satu-tahun-terakhir.html
Barr, T., Helms, C., Grant, K., & Messaoudi, I. (2016). Opposing Effects of Alcohol on the Immune System Overview of the Immune System HHS Public Access. Prog Neuropsychopharmacol Biol Psychiatry, 65, 242–251. https://doi.org/10.1016/j.pnpbp
Boule, L. A., & Kovacs, E. J. (2017). Alcohol, aging, and innate immunity. Journal of Leukocyte Biology, 102(1), 41–55. https://doi.org/10.1189/jlb.4ru1016-450r
Chaudhry, S. R., Luskin, V., & Panuganti, K. K. (2023). Anatomy, Abdomen and Pelvis, Spleen. In StatPearls. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK482235/
Das, S. (2023). Effects of high dose of long-term ethanol exposure on oxidative damage of spleen in experimental rat model. Indian Journal of Physiology and Allied Sciences, 75(04), 35–40. https://doi.org/10.55184/ijpas.v75i04.200
Hidayati, E., Berata, I. K., Samsuri, S., Sudimartini, L. M., & Merdana, I. M. (2018). Gambaran Histopatologi Limpa Tikus Putih yang Diberi Deksametason dan Vitamin E. Buletin Veteriner Udayana, 18. https://doi.org/10.24843/bulvet.2018.v10.i01.p03
Hidayati, S., Oktavianti, F., Susanti, D. A., & Aini, Q. (2022). Aktivitas Antiinflamasi In Vitro dan In Vivo Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.). Jurnal Sains Dan Kesehatan, 4(5), 488–494. https://doi.org/10.25026/jsk.v4i5.1195
Khan, R., Upaganlawar, A. B., & Upasani, C. (2014). Protective effects of Dioscorea Alata l. in aniline exposure-induced spleen toxicity in rats: A biochemical study. Toxicology International, 21(3), 294–299. https://doi.org/10.4103/0971-6580.155371
Kim, H. P., Ho Son, K., Chang, H. W., & Kang, S. S. (2004). Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms. J Pharmacol Sci, 96(3):229-45. doi: 10.1254/jphs.crj04003x.
Kurnianingtyas, E., Djati, M. S., & Rifa’i, M. (2013). Aktivitas Imunomodulator Polyscias obtusa Terhadap Sistem Imunitas Pada Bone Marrow Broiler Setelah Pemberian Salmonella typhimurium. Life Sci, 3(1).
Makiyah, S. N. N., Iszamriach, R., & Nofariyandi, A. (2014). Paparan Ultraviolet C Meningkatkan Diameter Pulpa Alba Limpa dan Indeks Mitotik Epidermis Kulit Mencit. Jurnal Kedokteran Brawijaya, 28(1), 17-21. https://doi.org/10.21776/ub.jkb.2014.028.01.4
Makiyah, S. N. N., & Djati, M. S. (2018). Potency of Purple Yam (Dioscorea alata) as an Immunomodulatory Agent. In Berkala Kedokteran, 14 (1), 89-98. http://dx.doi.org/10.20527/jbk.v14i1.4589
Makiyah, S. N. N., & Ni’mah, R. A. (2024). Umbi Uwi Ungu (Dioscorea alata L.) Aman dan Berefek Baik terhadap Organ Limpa. Monograf. Penerbit Madza. https://penerbitmadza.com/product/umbi-uwi-ungu-dioscorea-alata-l-aman-dan-berefek-baik-terhadap-organ-limpa/
Makiyah, S. N. N., & Wardhani, U. H. (2017). Potensi Ekstrak Etanol Buah Citrullus lanatus sebagai Agen Imunosupresi melalui Pengamatan Histologi Limpa Mencit BALB/c. Majalah Kedokteran Bandung, 49(4), 245–251. https://doi.org/10.15395/mkb.v49n4.1024
Meiliana, M. (2016). Pengaruh Pemberian Ekstrak Etanol Umbi Bawang Dayak (Eleuthrine Palmifolia (L.) Merr). Secara Oral pada Mencit BALB/c Terhadap Pencegahan Penurunan Jumlah NK Sel dan CD 8+. Jurnal Biosains Pascasarjana, 18(1), 13–23. https://doi.org/10.20473/jbp.v18i1.2016.13-23
Obidiegwu, J. E., Lyons, J. B., & Chilaka, C. A. (2020). The dioscorea genus (Yam)—An appraisal of nutritional and therapeutic potentials. In Foods (Vol. 9, Issue 9). MDPI AG. https://doi.org/10.3390/foods9091304
Perdana, P. G. R. W. (2021). Review Artikel: Aktivitas Imunomodulator Ekstrak Herba Meniran (Phyllanthus niruri L.). Jurnal Farmasi Malahayati. 4(1), 44-52 https://ejurnalmalahayati.ac.id/index.php/farmasi/article/view/4264/pdf
Putra, B., Azizah, R. N., & Nopriyanti, E. M. (2020). Efek Imunomodulator Ekstrak Etanol Herba Krokot (Portulaca oleracea L.) terhadap Tikus Putih (Rattus norvegicus) Jantan dengan Parameter Delayed Type Hypersensitivity (DTH). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(1), 20–25. https://doi.org/10.22487/j24428744.2020.v6.i1.14106
Ruiz-Cortes, K., Villageliu, D. N., & Samuelson, D. R. (2022). Innate lymphocytes: Role in alcohol-induced immune dysfunction. In Frontiers in Immunology (Vol. 13). Frontiers Media S.A. https://doi.org/10.3389/fimmu.2022.934617
Setiawan, H., Wulandari, S. W., & Fitriyani, A. N. (2021). Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar. Jurnal Veteriner, 22(4), 531–539. https://doi.org/10.19087/jveteriner.2021.22.4.531
Sulistyaningsih, E., Sa’diyah, N. A., Kusuma, I. F., Dewi, R., & Rachmania, S. (2024). Increased of Spleen White Pulp Diameter Post Dbl2β-Pfemp1 Recombinant Protein Injection in Wistar Rats: Pre-Clinical Study for Malaria Vaccine Development. Jurnal Profesi Medika: Jurnal Kedokteran Dan Kesehatan, 18(1), 51–57. https://doi.org/10.33533/jpm.v18i1.7451
WHO. (2022). Alcohol. https://www.who.int/news-room/fact-sheets/detail/alcohol
Zaputri, D. M., Wahdaniah, Triana, L., & Mujtahidah, ·. (2023). Uji Aktivitas Antiinflamasi Ekstrak Daun Bawang Dayak (Eleutherine Bulbosa (Mill.) Urb.) terhadap Stabilitas Membran Sel Darah Merah. Prosiding Rapat Kerja Nasional Asosiasi Institusi Perguruan Tinggi Teknologi Laboratorium Medik Indonesia. Volume 2.
Barr, T., Helms, C., Grant, K., & Messaoudi, I. (2016). Opposing Effects of Alcohol on the Immune System Overview of the Immune System HHS Public Access. Prog Neuropsychopharmacol Biol Psychiatry, 65, 242–251. https://doi.org/10.1016/j.pnpbp
Boule, L. A., & Kovacs, E. J. (2017). Alcohol, aging, and innate immunity. Journal of Leukocyte Biology, 102(1), 41–55. https://doi.org/10.1189/jlb.4ru1016-450r
Chaudhry, S. R., Luskin, V., & Panuganti, K. K. (2023). Anatomy, Abdomen and Pelvis, Spleen. In StatPearls. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK482235/
Das, S. (2023). Effects of high dose of long-term ethanol exposure on oxidative damage of spleen in experimental rat model. Indian Journal of Physiology and Allied Sciences, 75(04), 35–40. https://doi.org/10.55184/ijpas.v75i04.200
Hidayati, E., Berata, I. K., Samsuri, S., Sudimartini, L. M., & Merdana, I. M. (2018). Gambaran Histopatologi Limpa Tikus Putih yang Diberi Deksametason dan Vitamin E. Buletin Veteriner Udayana, 18. https://doi.org/10.24843/bulvet.2018.v10.i01.p03
Hidayati, S., Oktavianti, F., Susanti, D. A., & Aini, Q. (2022). Aktivitas Antiinflamasi In Vitro dan In Vivo Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.). Jurnal Sains Dan Kesehatan, 4(5), 488–494. https://doi.org/10.25026/jsk.v4i5.1195
Khan, R., Upaganlawar, A. B., & Upasani, C. (2014). Protective effects of Dioscorea Alata l. in aniline exposure-induced spleen toxicity in rats: A biochemical study. Toxicology International, 21(3), 294–299. https://doi.org/10.4103/0971-6580.155371
Kim, H. P., Ho Son, K., Chang, H. W., & Kang, S. S. (2004). Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms. J Pharmacol Sci, 96(3):229-45. doi: 10.1254/jphs.crj04003x.
Kurnianingtyas, E., Djati, M. S., & Rifa’i, M. (2013). Aktivitas Imunomodulator Polyscias obtusa Terhadap Sistem Imunitas Pada Bone Marrow Broiler Setelah Pemberian Salmonella typhimurium. Life Sci, 3(1).
Makiyah, S. N. N., Iszamriach, R., & Nofariyandi, A. (2014). Paparan Ultraviolet C Meningkatkan Diameter Pulpa Alba Limpa dan Indeks Mitotik Epidermis Kulit Mencit. Jurnal Kedokteran Brawijaya, 28(1), 17-21. https://doi.org/10.21776/ub.jkb.2014.028.01.4
Makiyah, S. N. N., & Djati, M. S. (2018). Potency of Purple Yam (Dioscorea alata) as an Immunomodulatory Agent. In Berkala Kedokteran, 14 (1), 89-98. http://dx.doi.org/10.20527/jbk.v14i1.4589
Makiyah, S. N. N., & Ni’mah, R. A. (2024). Umbi Uwi Ungu (Dioscorea alata L.) Aman dan Berefek Baik terhadap Organ Limpa. Monograf. Penerbit Madza. https://penerbitmadza.com/product/umbi-uwi-ungu-dioscorea-alata-l-aman-dan-berefek-baik-terhadap-organ-limpa/
Makiyah, S. N. N., & Wardhani, U. H. (2017). Potensi Ekstrak Etanol Buah Citrullus lanatus sebagai Agen Imunosupresi melalui Pengamatan Histologi Limpa Mencit BALB/c. Majalah Kedokteran Bandung, 49(4), 245–251. https://doi.org/10.15395/mkb.v49n4.1024
Meiliana, M. (2016). Pengaruh Pemberian Ekstrak Etanol Umbi Bawang Dayak (Eleuthrine Palmifolia (L.) Merr). Secara Oral pada Mencit BALB/c Terhadap Pencegahan Penurunan Jumlah NK Sel dan CD 8+. Jurnal Biosains Pascasarjana, 18(1), 13–23. https://doi.org/10.20473/jbp.v18i1.2016.13-23
Obidiegwu, J. E., Lyons, J. B., & Chilaka, C. A. (2020). The dioscorea genus (Yam)—An appraisal of nutritional and therapeutic potentials. In Foods (Vol. 9, Issue 9). MDPI AG. https://doi.org/10.3390/foods9091304
Perdana, P. G. R. W. (2021). Review Artikel: Aktivitas Imunomodulator Ekstrak Herba Meniran (Phyllanthus niruri L.). Jurnal Farmasi Malahayati. 4(1), 44-52 https://ejurnalmalahayati.ac.id/index.php/farmasi/article/view/4264/pdf
Putra, B., Azizah, R. N., & Nopriyanti, E. M. (2020). Efek Imunomodulator Ekstrak Etanol Herba Krokot (Portulaca oleracea L.) terhadap Tikus Putih (Rattus norvegicus) Jantan dengan Parameter Delayed Type Hypersensitivity (DTH). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(1), 20–25. https://doi.org/10.22487/j24428744.2020.v6.i1.14106
Ruiz-Cortes, K., Villageliu, D. N., & Samuelson, D. R. (2022). Innate lymphocytes: Role in alcohol-induced immune dysfunction. In Frontiers in Immunology (Vol. 13). Frontiers Media S.A. https://doi.org/10.3389/fimmu.2022.934617
Setiawan, H., Wulandari, S. W., & Fitriyani, A. N. (2021). Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar. Jurnal Veteriner, 22(4), 531–539. https://doi.org/10.19087/jveteriner.2021.22.4.531
Sulistyaningsih, E., Sa’diyah, N. A., Kusuma, I. F., Dewi, R., & Rachmania, S. (2024). Increased of Spleen White Pulp Diameter Post Dbl2β-Pfemp1 Recombinant Protein Injection in Wistar Rats: Pre-Clinical Study for Malaria Vaccine Development. Jurnal Profesi Medika: Jurnal Kedokteran Dan Kesehatan, 18(1), 51–57. https://doi.org/10.33533/jpm.v18i1.7451
WHO. (2022). Alcohol. https://www.who.int/news-room/fact-sheets/detail/alcohol
Zaputri, D. M., Wahdaniah, Triana, L., & Mujtahidah, ·. (2023). Uji Aktivitas Antiinflamasi Ekstrak Daun Bawang Dayak (Eleutherine Bulbosa (Mill.) Urb.) terhadap Stabilitas Membran Sel Darah Merah. Prosiding Rapat Kerja Nasional Asosiasi Institusi Perguruan Tinggi Teknologi Laboratorium Medik Indonesia. Volume 2.