Dioscorea alata enhances on the corpus luteum histology  in an endometriosis mouse model

Sri Nabawiyati Nurul Makiyah; Suci Nur Rahmawati

doi:10.35335/midwifery.v14i1.2301

Authors

Sri Nabawiyati Nurul Makiyah Universitas Muhammadiyah Yogyakarta, Indonesia
Suci Nur Rahmawati Universitas Muhammadiyah Yogyakarta, Indonesia

DOI:

https://doi.org/10.35335/midwifery.v14i1.2301

Keywords:

BALB/C Mouse, Corpus Luteum, Endometriosis, Ethanol Extract of Dioscorea Alata

Abstract

Endometriosis is a chronic inflammatory disease that causes ovarian damage and infertility. Dioscorea alata contains anti-inflammatory and antioxidant agents. This study examined the effect of ethanol extract of Dioscorea alata (EEDA) on the histology of corpus luteum in an endometriosis mouse model. This research is a quasi-experimental with a post-test-only controlled group design. Twenty-four mice were divided into six groups: control (C), negative control (NC) induced endometriosis model, positive control (PC) induced endometriosis model and letrozole, and three treatment groups induced endometriosis model and EEDA 50, 250, and 500 mg/kg body weight (T1, T2, T3). The number and diameter of corpus luteum analyzed with Kruskal-Wallis test, One-way ANOVA, and Duncan's test. The number of corpus luteum decreased in negative control group and increased significantly in the treatment groups (P = 0.038). The diameter of corpus luteum decreased in the negative control group and increased non-significantly in the treatment groups (P = 0.206). The conclusions of this research prove that EEDA has a positive effect both the number and diameter of corpus luteum in an endometriosis mouse model.

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References

Ali, U., Salman, A., Riaz, M., Hamid, S., Hanif, F., & Mansoor, S. (2024). Effects of Spearmint (Peppermint) on Morphological, Histological, and Biochemical Aspects of Ovaries. Journal of Allama Iqbal Medical College, 22(3), 100-106

Allaire, C., Bedaiwy, M. A., & Yong, P. J. (2023). Diagnosis and Management of Endometriosis. Canadian Medical Association Journal, 195 (10). https://doi.org/10.1503/cmaj.220637

Bangar, S. P., Dunno, K., Dhull, S. B., Kumar, S.A., Changan, S., Maqsood, S., & Rusu, A. V. (2022). Avocado Seed Discoveries: Chemical Composition, Biological Properties, and Industrial Food Applications. Food Chemistry. https://doi.org/10.1016/j.fochx.2022.100507

Barnard, N. D., Holtz, D. N., Schmidt, N., Kolipaka, S., Hata, E., Sutton, M., Znayenko-Miller, T., Hazen, N. D., Cobb, C., & Kahleova, H. (2023). Nutrition in the Prevention and Treatment of Endometriosis: A Review. Frontriers in Nutrition. https://doi.org/https://doi.org/10.3389/fnut.2023.1089891

Batubara, M. S., Sabri, E., & Tanjung, M. (2020). Pengaruh Pemberian Ekstrak Daun Andaliman (Zanthoxylum Acanthopodium Dc.) terhadap Histologis Ovarium Mencit (Mus Musculus L.). Jurnal Biologi Lingkungan Industri Kesehatan, 6(2), 196–209. https://doi.org/10.31289/biolink.v6i2.2409

Bonavina, G., & Taylor, H. S. (2022). Endometriosis-associated infertility: From pathophysiology to tailored treatment. In Frontiers in Endocrinology (Vol. 13). Frontiers Media S.A. https://doi.org/10.3389/fendo.2022.1020827

Brahmana, I. B., Pamungkasari, L. P., Majdawati, A., & Inayati, I. (2023). Sosialisasi Penggunaan Obat Herbal Sebagai Terapi Endometriosis. Jurnal Masyarakat Mandiri, 7(4), 3931. https://doi.org/10.31764/jmm.v7i4.15220

Brahmana, I. B., Soetrisno, S., Pangestu, M., Cempaka, R., & Puspitasari, I. (2025). Induction of Fresh Chocolate Cyst Pulp into Endometriosis Mice Model. Journal of Obstetrics, Gynecology and Cancer Research, 10(7), 532–536. https://doi.org/10.24200/jogcr.10.7.532

Cong, S., Peng, Q., Cao, L., Yi, Q., Liu, Y., Li, L., Tong, Q., & Liang, D. (2024). Diosgenin Prevents Periodontitis by Inhibiting Inflammation and Promoting Osteogenic Differentiation. Oral Diseases, 30(4), 2497–2510. https://doi.org/10.1111/odi.14708

Essono, S. M., Mvondo, M. A., Ngadjui, E., Kemka, N.F. X., & Watcho, P. (2020). The Ethanol Extract of Avocado (Persea americana Mill. (Lauraceae)) Seeds Successfully Induces Implant Regression and Restores Ovarian Dynamic in a Rat Model of Endometriosis. Evidence-Based Complementary and Alternative Medicine. https://doi.org/10.1155/2020/8521831

Feng, H., Shi, H., Yang, F., Yun, Y., & Wang, X. (2023). Impact of Anthocyanins Derived from Dioscorea alata L. on Growth Performance, Carcass Characteristics, Antioxidant Capacity, and Immune Function of Hainan Black Goats. Frontiers in Veterinary Science, 10. https://doi.org/10.3389/fvets.2023.1283947

Herrera, G. C., Salmun, N.S., Gascón, J., Méndez, A., Tienhoven, X. A., Martínez, M. F., Muleiro, A.M., Vasco R.M.X., López, T.M.F., Barroso, V.M. J., Noemi, T.I., Cruz, O.A., Bautista, G.M. F., Sarkis, N.J.A., Vélez, R.I., Murguiondo, P.R., Salazar, F. E., Sierra, B.A.G., Rivera, R.E.G., … Cabrera, C.R. (2024). Endometriosis: A Comprehensive Analysis of the Pathophysiology, Treatment, and Nutritional Aspects, and Its Repercussions on the Quality of Life of Patients. Biomedicines. https://doi.org/10.3390/biomedicines12071476

Hestianah, E. P., Widjiati, W., Ntoruru, J. M., & Widyanugraha, M. Y. A. (2024). Administration of Nanocurcumin in Mice Models of Endometriosis as an Effort to Improve Folliculogenesis. Biomedical and Pharmacology Journal, 17(2), 939–947. https://doi.org/10.13005/bpj/2914

Jamaludin, N. N., Mokhtar, A., Jamil, N. F. M., Muhammad, H., & Saad, W. M. M. (2023). Pharmacological Activities of Dioscorea spp.: A Narrative Review. Academi of Science Journal, 18, 1–19. https://doi.org/10.32802/ASMSCJ.2023.978

Latief, M., Fisesa, A.T., Sari,P.M., & Tarigan, I.L. (2021). Aktivitas Antiinflamasi Ekstrak Etanol Daun Sungkai (Peronema Canescens Jack) pada Mencit Terinduksi Karagenan. Jurnal Farmasi Sains dan Praktis, 7(2). http://journal.ummgl.ac.id/index.php/pharmacy

Makiyah, S. N. N., Kita, M., Setyawati, I., & Tasminatun, S. (2022). Dioscorea alata L. Tubers Improve Diabetes through Anti-hyperglycemia, Anti-inflammation, Ameliorate Insulin Resistance and Mitochondrial Dysfunction. The Indonesian Biomedical Journal, 14(4), 365–375. https://doi.org/10.18585/INABJ.V14I4.1966

Makiyah, S. N. N., Usman, S., & Adawiyah, R. (2026). Purple Yam (Dioscorea alata) Tuber Improves the Histological Appearance of the Ampulla of the Uterine Tube in a Mouse Model of Endometriosis. Bioscientist : Jurnal Ilmiah Biologi, 14(1), 225–238. https://doi.org/10.33394/bioscientist.v14i1.20007

Makiyah, S. N. N., & Zahra, A. N. (2025). The Immunomodulatory Activity of Dioscorea Alata on Spleen Histology of Rats Induced with 50% Alcohol. Science Midwifery, 13(1), 2721–9453.

Maulina, A. R., Monica, D. T., Meisya, D. Y., Jannah, M., Ahda, Y., & Atifah, Y. (2023). Pengaruh Pemberian Pare Hijau (Momordica Conchinchinensis) terhadap Reproduksi Mencit Betina (Mus Musculus). Jurnal Ilmu Kedokteran Dan Kesehatan, 10(9), 2549–4864. http://ejurnalmalahayati.ac.id/index.php/kesehatan

Momenimovahed, Z., Salehiniya, H., Allahqoli, L., Laganà, A. S., & Mazidimoradi, A. (2024). Effects of Herbal Compounds on Various Aspects of Endometriosis Treatment: A Systematic Review. European Review for Medical and Pharmalogical Science.